Comparison of HPLC-Chip/MS with conventional nanoflow LC/MS for proteomic analyses

Nanoflow LC/MS is commonly used for protein identification and biomarker discovery, but setting up and maintaining the intricate LC plumbing can be a challenge. The Agilent HPLC-Chip represents breakthrough technology that integrates nanoflow HPLC columns, associated connections, and a nanoelectrospray emitter on a single, small, reusable microfluidic chip. The HPLC-Chip and a companion HPLCChip/MS interface afford much better ease-of-use than traditional systems. This Application Note demonstrates performance of the HPLC-Chip/MS versus a state-of-the-art conventional nanoflow LC/MS. A direct comparison shows that the HPLC-Chip/MS system produces better chromatography and sensitivity, leading to more identified peptides and proteins. Application Note Martin Vollmer Christine Miller Georges L. Gauthier cerevisiae). This work will illustrate that the chip format delivers reduced sample losses and lower delay volumes, thus producing uncompromised chromatographic performance and more identified peptides and proteins. In sum, the HPLC-Chip/MS system provides better results than a state-of-theart conventional system, with much better ease-of-use and reliability.